Analyte-localization device for point-of-use processing of sub-millimetre areas on surfaces

SENSORS AND ACTUATORS B-CHEMICAL

卷:258

Article

Oskooei, Ali¹  Kaigala, Govind¹ 

[1] IBM Res Zurich, Saumerstr 4, CH-8803 Ruschlikon, Switzerland

关键词: Point-of-use;    Vacuum-operated analyte localization;    Micro-device;    Biomedical sensing;    Surface processing;    Tissue staining;   

DOI  :  10.1016/j.snb.2017.11.130

来源: Elsevier

【 摘 要 】

We present a portable, simple-to-operate, point-of-use analyte surface localization device. We take advantage of a set of hydrodynamic design features and components that achieve passive analyte localization by means of a single vacuum input. The vacuum source can be supplied by mechanical or battery-operated vacuum sources that are portable and allow point-of-use operation in the absence of electricity. We discuss the governing hydrodynamic principle and design parameters in detail. In a case study, we demonstrate the applicability of our technology through successful localization of a solution of rhodamine on a polydimethylsiloxane (PDMS) substrate and production of sub-millimetre-sized spots via application of a mild vacuum level of less than -10 kPa. In addition, using our devised technology, we demonstrate localized staining of human breast cancer cell blocks and human breast cancer tissue sections. (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

Free