Carbon nanodots based biosensors for gene mutation detection

SENSORS AND ACTUATORS B-CHEMICAL

卷:256

Article

Garcia-Mendiola, Tania^1,2,3  Bravo, Iria^1,2  Maria Lopez-Moreno, Jose¹  Pariente, Felix^1,2,3  Wannemacher, Reinhold²  Weber, Karina^4,5,6  Popp, Juergen^4,5,6  Lorenzo, Encarnacion^1,2,3 

[1] Univ Autonoma Madrid, Dept Quim Analit & Anal Instrumental, E-28049 Madrid, Spain

[2] Inst Madrileno Estudios Avanzados IMDEA Nanocienc, Faraday 9,Campus UAM, Madrid 28049, Spain

[3] Univ Autonoma Madrid, Inst Adv Res Chem Sci IAdChem, E-28049 Madrid, Spain

[4] Leibniz Inst Photon Technol IPHT, Albert Einstein Str 9, D-07745 Jena, Germany

[5] Friedrich Schiller Univ Jena, Inst Phys Chem, Helmholtzweg 4, D-07743 Jena, Germany

[6] Friedrich Schiller Univ Jena, Abbe Ctr Photon, Helmholtzweg 4, D-07743 Jena, Germany

关键词: Carbon nanodots;    DNA electrochemical biosensor;    Detection of CFTR gene mutation;   

DOI  :  10.1016/j.snb.2017.10.105

来源: Elsevier

【 摘 要 】

An electrochemical DNA biosensor based on a carbon nanodots (CDs) modified screen-printed gold electrode as a transducer is reported in this work. CDs were synthesized by thermal carbonization of ethyleneglycol bis-(2-aminoethylether)-N, N, N', N'-tetraacetic acid (EGTA) and characterized by different techniques (DLS, TEM, FTIR, Raman). The electrode surface modification was accomplished by dropcasting a suspension of CDs. SEM analysis and cyclic voltammetry were used to characterize the resulting modified electrode. Synthetic 25-mer or 100-mer DNA capture probes, capable to hybridize with a specific sequence of the pathogen Helicobacter pylori or the cystic fibrosis transmembrane regulator (CFTR) gene were attached to the CDs-gold surface. A 25-bases synthetic fully complementary sequence or a single nucleotide polymorphism to the DNA capture probe and a 373-bases PCR amplicon of exon 11 of CFTR containing a sequence complementary to the capture probe, were employed as target. The hybridization event was electrochemically monitored by using safranine as redox indicator, which selectively binds to double stranded DNA (dsDNA). A detection limit of 0.16 nM was obtained for the 25-mer synthetic target DNA. The biosensor shows a very high reproducibility and selectivity, allowing to detect a single nucleotide polymorphism. It has been applied to the detection of F508del mutation in the CFTR gene. (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

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