Electrochemical Detection of a Dengue-related Oligonucleotide Sequence Using Ferrocenium as a Hybridization Indicator

Sensors

Fernando Rodrigues Ribeiro Teles²  Duarte Miguel Fran๺ dos Prazeres¹ 

[1] IBB–Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001, Lisbon, Portugal. Tel.: [phone]; Fax: [phone]; E-mail:;Laboratório de Imunopatologia Keizo-Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego, S/N, Campus Universitário, Cidade Universitária, Recife, PE – CEP: 50670-901, Brazil. Tel.: [phone]; Fax: [phone]; E-mail:

关键词: modified glassy carbon electrode;    DNA electrochemical biosensor;    DNA immobilization;    hybridization indicator;    chitosan;   

DOI  :  10.3390/s7112510

来源: mdpi

【 摘 要 】

A simple method for electrochemical detection of a synthetic 20-bp oligonucleotide sequence related with dengue virus genome was developed. A complimentary DNA probe sequence was electrostatically immobilized onto a glassy carbon electrode modified with chitosan. Electrochemical detection of hybridization between probe and target was performed by cyclic voltammetry, using ferrocene (Fc⁺) as a hybridization label. After hybridization, the peak current response of Fc⁺ oxidation increased around 26%. A higher voltammetric decay rate constant (k_d) and a lower half-life period (t_1/2) for the interaction of Fc⁺ with dsDNA compared to those with ssDNA quantitatively characterize the different strengths of interaction with both types of DNA. By combining the simplicity of DNA immobilization onto a chitosan film and suitable voltammetric detection of hybridization concomitant with ferrocene attachment, a good discrimination between ssDNA and dsDNA was obtained.

【 授权许可】

Unknown   
© 2007 by MDPI (http://www.mdpi.org).