A novel fluorometric turn-on assay for lipase activity based on an aggregation-induced emission (AIE) luminogen

SENSORS AND ACTUATORS B-CHEMICAL

卷:238

Article

Shi, Jie¹  Zhang, Shan¹  Zheng, Mingming¹  Deng, Qianchun¹  Zheng, Chang¹  Li, Jing²  Huang, Fenghong¹ 

[1] Chinese Acad Agr Sci, Hubei Key Lab Lipid Chem & Nutr, Oil Crops Res Inst, Wuhan 430062, Peoples R China

[2] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China

关键词: Aggregation-induced emission;    Lipase activity assay;    Tetraphenylethene;    Hydrolysis reaction;    Fluorescence turn-on;   

DOI  :  10.1016/j.snb.2016.07.116

来源: Elsevier

【 摘 要 】

Lipase plays critical roles in the industrial catalysis, drug discovery and medical diagnosis of diseases. In this paper, based on the consideration that a tetraphenylethylene (TPE) derivative P1 with a-COOC6H13 group can be hydrolyzed into TPE-COOH which shows low solubility in same solutions and thus aggregation can occur easily, a novel fluorescence turn-on probe bearing aggregation-induced emission (AIE) characteristics to rapidly visualize lipase activities has been constructed with compound P1. Lipase of concentrations as low as 0.1 mg mL(-1) can be detected and the assay range of lipase was located in 0.1-1.3 mg mL(-1). On the other hand, the selectivity of probe P1, dynamic monitoring of enzymatic reaction and preliminary commercially enzyme activity screening was also summarized. (C) 2016 Elsevier B.V. All rights reserved.

【 授权许可】

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