Development of targeted STORM for super resolution imaging of biological samples using digital micro-mirror device

OPTICS COMMUNICATIONS

卷:404

Article

Peedikakkal, Liyana Valiya¹  Steventon, Victoria^2,3  Furley, Andrew^2,3  Cadby, Ashley J.¹ 

[1] Dept Phys & Astron, Hicks Bldg,Howtsfield Rd, Sheffield S3 7RH, S Yorkshire, England

[2] Univ Sheffield, Bateson Ctr, Sheffield S10 2TN, S Yorkshire, England

[3] Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, England

关键词: Localization microscopy;    Digital mirror device;    Super-resolution;    STORM;   

DOI  :  10.1016/j.optcom.2017.06.055

来源: Elsevier

【 摘 要 】

We demonstrate a simple illumination system based on a digital mirror device which allows for fine control over the power and pattern of illumination. We apply this to localization microscopy (LM), specifically stochastic optical reconstruction microscopy (STORM). Using this targeted STORM, we were able to image a selected area of a labelled cell without causing photo-damage to the surrounding areas of the cell. (C) 2017 The Authors. Published by Elsevier B.V.

【 授权许可】

Free