High resolution optical DNA mapping

Super-resolution;optical DNA mapping;genomic variations;copy-number variations;Single molecule-High Resolution Imaging with Photobleaching (SHRImP);High-Resolution Co-localization (SHREC);Deoxyribonucleic Acid (DNA)

Baday, Murat

关键词: Super-resolution;    optical DNA mapping;    genomic variations;    copy-number variations;    Single molecule-High Resolution Imaging with Photobleaching (SHRImP);    High-Resolution Co-localization (SHREC);    Deoxyribonucleic Acid (DNA);   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/44261/Murat_Baday.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Many types of diseases including cancer and autism are associated with copy-number variations in the genome. Most of these variations could not be identified with existing sequencing and optical DNA mapping methods. We have developed Multi-color Super-resolution technique, with potential for high throughput and low cost, which can allow us to recognize more of these variations. Our technique has made 10–fold improvement in the resolution of optical DNA mapping.Using a 180 kb BAC clone as a model system, we resolved dense patterns from 108 fluorescent labels of two different colors representing two different sequence-motifs. Overall, a detailed DNA map with 100 bp resolution was achieved, which has the potential to reveal detailed information about genetic variance and to facilitate medical diagnosis of genetic disease.