Fluorescence nanoscopy. Methods and applications

Journal of Chemical Biology

Jose Requejo-Isidro¹ 

[1] Unidad de Biofísica, CSIC-UPV/EHU, Barrio de Sarriena, s/n., 48940 Leioa, Spain

关键词: Super-resolution;    Fluorescence nanoscopy;    Subdiffraction imaging;    STED;    STORM;    PALM;    SIM;   

DOI  :  10.1007/s12154-013-0096-3

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

Fluorescence nanoscopy refers to the experimental techniques and analytical methods used for fluorescence imaging at a resolution higher than conventional, diffraction-limited, microscopy. This review explains the concepts behind fluorescence nanoscopy and focuses on the latest and promising developments in acquisition techniques, labelling strategies to obtain highly detailed super-resolved images and in the quantitative methods to extract meaningful information from them.

【 授权许可】

Unknown