Depth-encoded spectral domain phase microscopy for simultaneous multi-site nanoscale optical measurements

OPTICS COMMUNICATIONS

卷:284

Article

Hendargo, Hansford C.¹  Bower, Bradley A.¹  Reinstein, Alex S.¹  Shepherd, Neal²  Tao, Yuankai K.¹  Izatt, Joseph A.¹ 

[1] Duke Univ, Dept Biomed Engn, Durham, NC 27708 USA

[2] Duke Univ, Med Ctr, Dept Pediat, Jean & George Brumley Jr Neonatal Perinatal Res I, Durham, NC 27710 USA

关键词: Phase microscopy;    Optical coherence tomography;    Interferometry;    Nanoscale measurement;   

DOI  :  10.1016/j.optcom.2011.06.001

来源: Elsevier

【 摘 要 】

Spectral domain phase microscopy (SDPM) is an extension of spectral domain optical coherence tomography (SDOCT) that exploits the extraordinary phase stability of spectrometer-based systems with common-path geometry to resolve sub-wavelength displacements within a sample volume. This technique has been implemented for high resolution axial displacement and velocity measurements in biological samples, but since axial displacement information is acquired serially along the lateral dimension, it has been unable to measure fast temporal dynamics in extended samples. Depth-encoded SDPM (DESDPM) uses multiple sample arms with unevenly spaced common path reference reflectors to multiplex independent SDPM signals from separate lateral positions on a sample simultaneously using a single interferometer, thereby reducing the time required to detect unique optical events to the integration period of the detector. Here, we introduce DESDPM and demonstrate the ability to acquire useful phase data concurrently at two laterally separated locations in a phantom sample as well as a biological preparation of spontaneously beating chick cardiomyocytes. DESDPM may be a useful tool for imaging fast cellular phenomena such as nervous conduction velocity or contractile motion. (C) 2011 Elsevier B.V. All rights reserved.

【 授权许可】

Free