A method for assessing the fidelity of optical diffraction tomography reconstruction methods using structured illumination

OPTICS COMMUNICATIONS

卷:454

Article

Ayoub, Ahmed B.¹  Pham, Thanh-an²  Lim, Joowon¹  Unser, Michael²  Psaltis, Demetri¹ 

[1] Ecole Polytech Fed Lausanne, Opt Lab, CH-1015 Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, Biomed Imaging Grp, CH-1015 Lausanne, Switzerland

关键词: Optics;    Structured illumination;    Optical tomography;    3D refractive index reconstructions;    Biological samples;   

DOI  :  10.1016/j.optcom.2019.124486

来源: Elsevier

【 摘 要 】

A fundamental challenge in 3D tomographic reconstruction is the difficulty of obtaining ground truth about test objects with which to assess the fidelity of the 3D reconstruction. We present a method that provides such a quantitative metric of the accuracy of the three dimensional reconstruction for optical tomography. The method relies on spatial light modulation of the illumination beam through the sample and numerical back-propagation of the phase conjugated experimentally measured optical field through the 3D reconstruction of the object. The fidelity of this reconstruction provides a quantitative measure of the accuracy of the 3D reconstruction without direct access to the ground truth about the 3D object.

【 授权许可】

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