Compact and low-cost structured illumination microscopy using an optical fiber coupler

OPTICS COMMUNICATIONS

卷:436

Article

Hu, Shiming^1,2  Liu, Lei^1,2  Huang, Yizheng^1,2  Liu, Wenwen¹  Wei, Qingquan¹  Tan, Manqing^1,2  Yu, Yude^1,2 

[1] Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, Beijing 100083, Peoples R China

[2] Univ Chinese Acad Sci, Coll Mat Sci & Optoelect Technol, Beijing 100049, Peoples R China

关键词: Imaging systems;    Microscopy;    Superresolution;    Structured illumination;   

DOI  :  10.1016/j.optcom.2018.12.035

来源: Elsevier

【 摘 要 】

In this paper, a compact and low-cost structured illumination microscope (SIM) based on a 2 x 2 fiber coupler is presented. Fringe illumination is achieved by placing two output fiber tips at a conjugate Fourier plane of the sample plane as the point sources. Raw structured illumination (SI) images in different pattern orientations are captured when rotating the fiber mount. Following this, high resolution images are reconstructed from no-phaseshift raw SI images by using a joint Richardson-Lucy (jRL) deconvolution algorithm. Compared with other SIM setups, our method provides a much shorter illumination path, high power efficiency, and low cost.

【 授权许可】

Free