Structured illumination microscopy based on fiber devices

OPTICS COMMUNICATIONS

卷:460

Article

Hu, Shiming^1,2  Liu, Wenwen¹  Jie, Junyao^1,2  Huang, Yizheng^1,2  Wei, Qingquan¹  Tan, Manqing^1,2  Yu, Yude^1,2 

[1] Chinese Acad Sci, Inst Semicond, State Key Lab Integrated Optoelect, Beijing 100083, Peoples R China

[2] Univ Chinese Acad Sci, Coll Mat Sci & Optoelect Technol, Beijing 100049, Peoples R China

关键词: Imaging systems;    Microscopy;    Structured illumination;    Superresolution;    Optical section;   

DOI  :  10.1016/j.optcom.2019.125099

来源: Elsevier

【 摘 要 】

We present a simple and compact approach of structured illumination microscopy by using three 2 x 2 fiber couplers and one 1 x 4 MEMS optics switch. One uniform and three fringe illumination patterns were produced by placing seven output fiber tips at the conjugate Fourier plane of the illumination path. Stable and relatively high-speed illumination switching was achieved by the optics switch. Super-resolution and optical sectioned information was reconstructed from 4-frame data by using algorithms based on a joint Richardson-Lucy deconvolution method and a Hilbert transform method. By directly removing the out-focus components from the raw images provides better imaging results.

【 授权许可】

Free