Fourier analysis and synthesis tomography.

Wagner, Kelvin H. (University of Colorado at Boulder, Boulder, CO) ; Sinclair, Michael B. ; Feldkuhn, Daniel (University of Colorado at Boulder, Boulder, CO)

关键词: FOURIER ANALYSIS;    ILLUMINANCE;    OPTICAL SYSTEMS;    RESOLUTION;    TOMOGRAPHY;    TRANSFER FUNCTIONS;    PERFORMANCE Optical materials.;    Optical instruments-Testing.;    Microscopy-Technique.;    Optical tomography;   

DOI  :  10.2172/992332
RP-ID  :  SAND2010-3258
PID  :  OSTI ID: 992332
Others  :  TRN: US201022%%363

学科分类：工程和技术（综合）

美国|英语

来源: SciTech Connect

【 摘 要 】

Most far-field optical imaging systems rely on a lens and spatially-resolved detection to probe distinct locations on the object. We describe and demonstrate a novel high-speed wide-field approach to imaging that instead measures the complex spatial Fourier transform of the object by detecting its spatially-integrated response to dynamic acousto-optically synthesized structured illumination. Tomographic filtered backprojection is applied to reconstruct the object in two or three dimensions. This technique decouples depth-of-field and working-distance from resolution, in contrast to conventional imaging, and can be used to image biological and synthetic structures in fluoresced or scattered light employing coherent or broadband illumination. We discuss the electronically programmable transfer function of the optical system and its implications for imaging dynamic processes. Finally, we present for the first time two-dimensional high-resolution image reconstructions demonstrating a three-orders-of-magnitude improvement in depth-of-field over conventional lens-based microscopy.