A comparative proteomic study on the effects of metal pollution in oysters Crassostrea hongkongensis

MARINE POLLUTION BULLETIN

卷:112

Article

Xu, Lanlan^1,2  Ji, Chenglong¹  Wu, Huifeng¹  Tan, Qiaoguo³  Wang, Wen-Xiong⁴ 

[1] Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Xiamen Univ, Coll Environm & Ecol, Key Lab Coastal & Wetland Ecosyst, Minist Educ, Xiamen 361102, Peoples R China

[4] Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China

关键词: Metal pollution;    Crassostrea hongkongensis;    Hepatopancreas;    Proteomics;   

DOI  :  10.1016/j.marpolbul.2016.07.009

来源: Elsevier

【 摘 要 】

The metal pollution has posed great risk on the coastal organisms along the Jiulongjiang Estuary in South China. In this work, two-dimensional electrophoresis-based proteomics was applied to the oysters Crassostrea hongkongensis from metal pollution sites to characterize the proteomic responses to metal pollution. Metal accumulation and proteomic responses indicated that the oysters from BJ site were more severely contaminated than those from FG site. Compared with those oyster samples from the clean site (JZ), metal pollution induced cellular injuries, oxidative and immune stresses in oyster heapatopancreas from both BJ and FG sites via differential metabolic pathways. In addition, metal pollution in BJ site induced disturbance in energy and lipid metabolisms in oysters. Results indicated that cathepsin L and ferritin GF1 might be the biomarkers of As and Fe in oyster C. hongkongensis, respectively. This study demonstrates that proteomics is a useful tool for investigating biological effects induced by metal pollution. (C) 2016 Elsevier Ltd. All rights reserved.

【 授权许可】

Free