A remarkably diverse and well-organized virus community in a filter-feeding oyster

Microbiome

Research

Meng Wang¹  Peng Zhu²  Min Liu²  Hong-Ying Wei³  Yi-Fei Fang⁴  Tao Jin⁵  Wen-Guang Yuan⁶  Jie Lu⁷  Dian-Chang Zhang⁷  Tuo Yao⁷  Ling-Tong Ye⁷  Jing-Zhe Jiang⁸  Mang Shi⁹  Ming Duan^1,10  Shao-Kun Shi^1,11  Ying-Xiang Guo^1,12  Li-Ling Yang^1,12 

[1] Bureau of Agriculture and Rural Affairs of Conghua District, 510925, Guangzhou, Guangdong, China;College of Fisheries and Life Science, Shanghai Ocean University, 201306, Shanghai, China;College of Fisheries and Life Science, Shanghai Ocean University, 201306, Shanghai, China;Guangdong Magigene Biotechnology Co Ltd, 510000, Guangzhou, Guangdong, China;College of Fisheries and Life Science, Shanghai Ocean University, 201306, Shanghai, China;State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, Hubei, China;Guangdong Magigene Biotechnology Co Ltd, 510000, Guangzhou, Guangdong, China;Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, 510006, Guangzhou, Guangdong, China;Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, Guangdong, China;Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510300, Guangzhou, Guangdong, China;College of Fisheries and Life Science, Shanghai Ocean University, 201306, Shanghai, China;Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, 510006, Guangzhou, Guangdong, China;Tianjin Agricultural University, 300384, Tianjin, China;School of Medicine, Sun Yat-Sen University, 518107, Shenzhen, Guangdong, China;Shanghai Majorbio Bio-Pharm Technology Co Ltd, 201203, Shanghai, China;Shenzhen Fisheries Development Research Center, 518067, Shenzhen, Guangdong, China;Tianjin Agricultural University, 300384, Tianjin, China;

关键词: Crassostrea hongkongensis;    Bivalve;    Mollusk;    Metagenome;    Circovirus;    Viral-like particle enrichment;    Mussel Watch;    Multiple displacement amplification;   

DOI  :  10.1186/s40168-022-01431-8

 received in 2022-02-09, accepted in 2022-11-17,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundViruses play critical roles in the marine environment because of their interactions with an extremely broad range of potential hosts. Many studies of viruses in seawater have been published, but viruses that inhabit marine animals have been largely neglected. Oysters are keystone species in coastal ecosystems, yet as filter-feeding bivalves with very large roosting numbers and species co-habitation, it is not clear what role they play in marine virus transmission and coastal microbiome regulation.ResultsHere, we report a Dataset of Oyster Virome (DOV) that contains 728,784 nonredundant viral operational taxonomic unit contigs (≥ 800 bp) and 3473 high-quality viral genomes, enabling the first comprehensive overview of both DNA and RNA viral communities in the oyster Crassostrea hongkongensis. We discovered tremendous diversity among novel viruses that inhabit this oyster using multiple approaches, including reads recruitment, viral operational taxonomic units, and high-quality virus genomes. Our results show that these viruses are very different from viruses in the oceans or other habitats. In particular, the high diversity of novel circoviruses that we found in the oysters indicates that oysters may be potential hotspots for circoviruses. Notably, the viruses that were enriched in oysters are not random but are well-organized communities that can respond to changes in the health state of the host and the external environment at both compositional and functional levels.ConclusionsIn this study, we generated a first “knowledge landscape” of the oyster virome, which has increased the number of known oyster-related viruses by tens of thousands. Our results suggest that oysters provide a unique habitat that is different from that of seawater, and highlight the importance of filter-feeding bivalves for marine virus exploration as well as their essential but still invisible roles in regulating marine ecosystems.APUP9ceFw5rgMggB2pWS5VVideo Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

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