The purplish bifurcate mussel Mytilisepta virgata gene expression atlas reveals a remarkable tissue functional specialization

BMC Genomics

Research Article

Francesca Spazzali¹  Marco Gerdol¹  Alberto Pallavicini¹  Yasuhiro Ozeki²  Imtiaj Hasan³  Hideaki Fujita⁴  Shunsuke Shimojo⁴  Toru Koike⁴  Yuki Fujii⁴  Kaname Yamamoto⁴ 

[1] Department of Life Sciences, University of Trieste, Via Giorgieri 5, 34126, Trieste, Italy;Department of Life and Environmental System Science, Graduate School of NanoBio Sciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, 236-0027, Yokohama, Japan;Department of Life and Environmental System Science, Graduate School of NanoBio Sciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, 236-0027, Yokohama, Japan;Department of Biochemistry and Molecular Biology, Faculty of Science, University of Rajshahi, 6205, Rajshahi, Bangladesh;Department of Pharmacy, Faculty of Pharmaceutical Science, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, 859-3298, Nagasaki, Japan;

关键词: Mussel;    Transcriptome;    RNA-seq;    Lectins;    Phylogenomics;    Bivalve;    Mollusk;   

DOI  :  10.1186/s12864-017-4012-z

 received in 2017-04-06, accepted in 2017-08-02,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMytilisepta virgata is a marine mussel commonly found along the coasts of Japan. Although this species has been the subject of occasional studies concerning its ecological role, growth and reproduction, it has been so far almost completely neglected from a genetic and molecular point of view. In the present study we present a high quality de novo assembled transcriptome of the Japanese purplish mussel, which represents the first publicly available collection of expressed sequences for this species.ResultsThe assembled transcriptome comprises almost 50,000 contigs, with a N50 statistics of ~1 kilobase and a high estimated completeness based on the rate of BUSCOs identified, standing as one of the most exhaustive sequence resources available for mytiloid bivalves to date. Overall this data, accompanied by gene expression profiles from gills, digestive gland, mantle rim, foot and posterior adductor muscle, presents an accurate snapshot of the great functional specialization of these five tissues in adult mussels.ConclusionsWe highlight that one of the most striking features of the M. virgata transcriptome is the high abundance and diversification of lectin-like transcripts, which pertain to different gene families and appear to be expressed in particular in the digestive gland and in the gills. Therefore, these two tissues might be selected as preferential targets for the isolation of molecules with interesting carbohydrate-binding properties.In addition, by molecular phylogenomics, we provide solid evidence in support of the classification of M. virgata within the Brachidontinae subfamily. This result is in agreement with the previously proposed hypothesis that the morphological features traditionally used to group Mytilisepta spp. and Septifer spp. within the same clade are inappropriate due to homoplasy.

【 授权许可】

CC BY   
© The Author(s). 2017