Glial cell line-derived neurotrophic factor and antioxidants preserve the electrical responsiveness of the spiral ganglion neurons after experimentally induced deafness

NEUROBIOLOGY OF DISEASE

卷:29

Article

Maruyama, Jun^1,2,3  Miller, Josef M.^1,2,4  Ulfendahl, Mats^1,2,5 

[1] Karolinska Inst, Ctr Hearing & Commun Res, S-10401 Stockholm, Sweden

[2] Karolinska Inst, Dept Clin Neurosci, S-10401 Stockholm, Sweden

[3] Ehime Univ, Sch Med, Dept Otolaryngol, Matsuyama, Ehime, Japan

[4] Univ Michigan, Kresge Hearing Res Inst, Ann Arbor, MI 48109 USA

[5] Karolinska Univ Hosp Solna, Dept Otolaryngol, Stockholm, Sweden

关键词: antioxidants;    GDNF;    spiral ganglion;    cochlea;    inner ear;    neurotrophic factor;    intervention;    deafferentation;    electrical;    auditory;    brainstem response;   

DOI  :  10.1016/j.nbd.2007.07.026

来源: Elsevier

【 摘 要 】

Cochlear implant surgery is currently the therapy of choice for profoundly deaf patients. However, the functionality of cochlear implants depends on the integrity of the auditory spiral ganglion neurons. This study assesses the combined efficacy of two classes of agents found effective in preventing degeneration of the auditory nerve following deafness, neurotrophic factors, and antioxidants. Guinea pigs were deafened and treated for 4 weeks with either local administration of GDNF or a combination of GDNF and systemic injections of the antioxidants ascorbic acid and Trolox. The density of surviving spiral ganglion cells was significantly enhanced and the thresholds for eliciting an electrically evoked brain stem response were significantly reduced in GDNF treated animals compared to deafened-untreated. The addition of antioxidants significantly enhanced the evoked responsiveness over that observed with GDNF alone. The results suggest multiple sites of intervention in the rescue of these cells from deafferentation-induced cell death. (c) 2007 Elsevier Inc. All rights reserved.

【 授权许可】

Free