Comparisons of dual isogenic human iPSC pairs identify functional alterations directly caused by an epilepsy associated SCN1A mutation

NEUROBIOLOGY OF DISEASE

卷:134

Article

Xie, Yunyao¹  Ng, Nathan N.^1,5  Safrina, Olga S.¹  Ramos, Carmen M.¹  Ess, Kevin C.²  Schwartz, Philip H.³  Smith, Martin A.⁴  O'Dowd, Diane K.¹ 

[1] Univ Calif Irvine, Dept Dev & Cell Biol, Irvine, CA 92717 USA

[2] Vanderbilt Univ, Med Ctr, Dept Pediat, Nashville, TN 37232 USA

[3] Childrens Hosp, Orange Cty Res Inst, Orange, CA USA

[4] Univ Calif Irvine, Dept Anat & Neurobiol, Irvine, CA 92717 USA

[5] Stanford Univ, Sch Med, Stanford, CA 94305 USA

关键词: Isogenic;    Induced pluripotent stem cells;    Neurons;    SCN1A;    Genetic epilepsy with febrile seizure plus;    Electrophysiology;    Disease modeling;   

DOI  :  10.1016/j.nbd.2019.104627

来源: Elsevier

【 摘 要 】

Over 1250 mutations in SCN1A, the Navl.1 voltage-gated sodium channel gene, are associated with seizure disorders including GEES +. To evaluate how a specific mutation, independent of genetic background, causes seizure activity we generated two pairs of isogenic human iPSC lines by CRISPR/Cas9 gene editing. One pair is a control line from an unaffected sibling, and the mutated control carrying the GEFS+ K1270T SCN1A mutation. The second pair is a GEFS+ patient line with the K1270T mutation, and the corrected patient line. By comparing the electrophysiological properties in inhibitory and excitatory iPSC-derived neurons from these pairs, we found the K1270T mutation causes cell type-specific alterations in sodium current density and evoked firing, resulting in hyperactive neural networks. We also identified differences associated with genetic background and interaction between the mutation and genetic background. Comparisons within and between dual pairs of isogenic iPSC-derived neuronal cultures provide a novel platform for evaluating cellular mechanisms underlying a disease phenotype and for developing patient-specific anti-seizure therapies.