Using induced pluripotent stem cell neuronal models to study neurodegenerative diseases

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1866

Review

Zhang, Xinwen^1,2  Hu, Di²  Shang, Yutong²  Qi, Xin^2,3 

[1] China Med Univ, Ctr Implant Dent, Sch Stomatol, Shenyang 110002, Peoples R China

[2] Case Western Reserve Univ, Sch Med, Dept Physiol & Biophys, 10900 Euclid Ave,E516, Cleveland, OH 44106 USA

[3] Case Western Reserve Univ, Sch Med, Ctr Mitochondrial Dis, Cleveland, OH 44106 USA

关键词: Induced pluripotent stem cells;    Neurons;    Neurodegeneration;    Therapeutic development;    Neurodegenerative disease;    Disease modeling;   

DOI  :  10.1016/j.bbadis.2019.03.004

来源: Elsevier

【 摘 要 】

Current application of human induced pluripotent stem cells (hiPSCs) technology in patient-specific models of neurodegenerative disorders recapitulate some of key phenotypes of diseases, representing disease-specific cellular modeling and providing a unique platform for therapeutics development. We review recent efforts toward advancing hiPSCs-derived neuronal cell types and highlight their potential use for the development of more complex in vitro models of neurodegenerative diseases by focusing on Alzheimer's disease, Parkinson's disease, Huntington's disease and Amyotrophic lateral sclerosis. We present evidence from previous works on the important phenotypic changes of various neuronal types in these neurological diseases. We also summarize efforts on conducting low- and high-throughput screening experiments with hiPSCs toward developing potential therapeutics for treatment of neurodegenerative diseases. Lastly, we discuss the limitations of hiPSCs culture system in studying neurodegenerative diseases and alternative strategies to overcome these hurdles.

【 授权许可】

Free