Multiple genetic pathways regulating lifespan extension are neuroprotective in a G2019S LRRK2 nematode model of Parkinson ' s disease

NEUROBIOLOGY OF DISEASE

卷:151

Article

Senchuk, Megan M.¹  Van Raamsdonk, Jeremy M.^2,3,4  Moore, Darren J.¹ 

[1] Van Andel Inst, Dept Neurodegenerat Sci, Grand Rapids, MI 49503 USA

[2] McGill Univ, Dept Neurol & Neurosurg, Montreal, PQ H4A 3J1, Canada

[3] McGill Univ Hlth Ctr, Brain Repair & Integrat Neurosci Program, Metab Disorders & Complicat Program, Res Inst, Montreal, PQ H4A 3J1, Canada

[4] Harvard Med Sch, Dept Genet, Cambridge, MA 02115 USA

关键词: LRRK2;    Lifespan;    Neuroprotection;    Neurodegeneration;    Insulin;    Signaling;    Kinase;    Nematode;   

DOI  :  10.1016/j.nbd.2021.105267

来源: Elsevier

【 摘 要 】

Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene are the most frequent cause of late-onset, familial Parkinson's disease (PD), and LRRK2 variants are associated with increased risk for sporadic PD. While advanced age represents the strongest risk factor for disease development, it remains unclear how different age-related pathways interact to regulate LRRK2-driven late-onset PD. In this study, we employ a C. elegans model expressing PD-linked G2019S LRRK2 to examine the interplay between age-related pathways and LRRK2induced dopaminergic neurodegeneration. We find that multiple genetic pathways that regulate lifespan extension can provide robust neuroprotection against mutant LRRK2. However, the level of neuroprotection does not strictly correlate with the magnitude of lifespan extension, suggesting that lifespan can be experimentally dissociated from neuroprotection. Using tissue-specific RNAi, we demonstrate that lifespan-regulating pathways, including insulin/insulin-like growth factor-1 (IGF-1) signaling, target of rapamycin (TOR), and mitochondrial respiration, can be directly manipulated in neurons to mediate neuroprotection. We extend this finding for AGE1/PI3K, where pan-neuronal versus dopaminergic neuronal restoration of AGE-1 reveals both cell-autonomous and non-cell-autonomous neuroprotective mechanisms downstream of insulin signaling. Our data demonstrate the importance of distinct lifespan-regulating pathways in the pathogenesis of LRRK2-linked PD, and suggest that extended longevity is broadly neuroprotective via the actions of these pathways at least in part within neurons. This study further highlights the complex interplay that occurs between cells and tissues during organismal aging and disease manifestation.