An ALS-linked mutation in TDP-43 disrupts normal protein interactions in the motor neuron response to oxidative stress

NEUROBIOLOGY OF DISEASE

卷:144

Article

Feneberg, Emily¹  Gordon, David¹  Thompson, Alexander G.¹  Finelli, Mattea J.¹  Dafinca, Ruxandra¹  Candalija, Ana¹  Charles, Philip D.²  Mager, Imre³  Wood, Matthew J.³  Fischer, Roman²  Kessler, Benedikt M.²  Gray, Elizabeth¹  Turner, Martin R.¹  Talbot, Kevin¹ 

[1] Univ Oxford, Nuffield Dept Clin Neurosci, Oxford OX3 9DU, England

[2] Univ Oxford, Target Discovery Inst, Nuffield Dept Med, Oxford OX3 7FZ, England

[3] Univ Oxford, Dept Paediat, Med Sci Div, Oxford OX3 9DU, England

关键词: Amyotrophic lateral sclerosis;    TDP-43;    Neurodegeneration;    Oxidative stress;    Interactome;    Biomarker;    Motor neuron;   

DOI  :  10.1016/j.nbd.2020.105050

来源: Elsevier

【 摘 要 】

TDP-43 pathology is a key feature of amyotrophic lateral sclerosis (ALS), but the mechanisms linking TDP-43 to altered cellular function and neurodegeneration remain unclear. We have recently described a mouse model in which human wild-type or mutant TDP-43 are expressed at low levels and where altered stress granule formation is a robust phenotype of TDP-43(M337V/)- expressing cells. In the present study we use this model to investigate the functional connectivity of human TDP-43 in primary motor neurons under resting conditions and in response to oxidative stress. The interactome of human TDP-43(WT) or TDP-43(M337V) was compared by mass spectrometry, and gene ontology enrichment analysis identified pathways dysregulated by the M337V mutation. We found that under normal conditions the interactome of human TDP-43(WT) was enriched for proteins involved in transcription, translation and poly(A)-RNA binding. In response to oxidative stress, TDP-43(WT) recruits proteins of the endoplasmic reticulum and endosomal-extracellular transport pathways, interactions which are reduced in the presence of the M337V mutation. Specifically, TDP-43(M337V) impaired protein-protein interactions involved in stress granule formation including reduced binding to the translation initiation factors Poly(A)-binding protein and Eif4a1 and the endoplasmic reticulum chaperone Grp78. The M337V mutation also affected interactions involved in endosomal-extracellular transport and this this was associated with reduced extracellular vesicle secretion in primary motor neurons from TDP-43(M337)(V/-) mice and in human iPSCs-derived motor neurons. Taken together, our analysis highlights a TDP-43 interaction network in motor neurons and demonstrates that an ALS associated mutation may alter the interactome to drive aberrant pathways involved in the pathogenesis of ALS.