Control of Aβ release from human neurons by differentiation status and RET signaling

NEUROBIOLOGY OF AGING

卷:34

Article

Scholz, Diana¹  Chernyshova, Yana¹  Leist, Marcel¹ 

[1] Univ Konstanz, Dept Biol, D-78457 Constance, Germany

关键词: LUHMES;    Alzheimer's disease;    A beta;    RET;    GDNF;   

DOI  :  10.1016/j.neurobiolaging.2012.03.012

来源: Elsevier

【 摘 要 】

Few studies have compared the processing of endogenous human amyloid precursor protein (APP) in younger and older neurons. Here, we characterized LUHMES cells as a human model to study Alzheimer's disease-related processes during neuronal maturation and aging. Differentiated LUHMES expressed and spontaneously processed APP via the secretase pathways, and they secreted amyloid beta (A beta) peptide. This was inhibited by cholesterol depletion or secretase inhibition, but not by block of tau phosphorylation. In vitro aged cells increased A beta secretion without upregulation of APP or secretases. We identified the medium constituent glial cell line-derived neurotrophic factor (GDNF) as responsible for this effect. GDNF-triggered A beta release was associated with rapid upregulation of the GDNF coreceptor rearranged during transfection (RET). Other direct (neurturin) or indirect (nerve growth factor) RET activators also increased A beta, whereas different neurotrophins were ineffective. Downstream of RET, we found activation of protein kinase B (AKT) to be involved. Accordingly, inhibitors of the AKT regulator phosphatidylinositol-3-kinase completely blocked GDNF-triggered AKT phosphorylation and A beta increase. This suggests that RET signaling affects A beta release from aging neurons. (C) 2013 Elsevier Inc. All rights reserved.

【 授权许可】

Free