Cheek cell-derived α-synuclein and DJ-1 do not differentiate Parkinson's disease from control

NEUROBIOLOGY OF AGING

卷:35

Article

Stewart, Tessandra¹  Sui, Yu-Ting¹  Gonzalez-Cuyar, Luis F.¹  Wong, David T. W.²  Akin, David M.²  Tumas, Vitor⁷  Aasly, Jan⁸  Ashmore, Emily⁴  Aro, Patrick¹  Ginghina, Carmen¹  Korff, Ane¹  Zabetian, Cyrus P.^3,4  Leverenz, James B.³  Shi, Min^5,6  Zhang, Jing¹ 

[1] Univ Washington, Sch Med, Dept Pathol, Seattle, WA 98104 USA

[2] Univ Calif Los Angeles, Sch Dent, Los Angeles, CA 90024 USA

[3] Univ Washington, Sch Med, Dept Neurol, Seattle, WA 98104 USA

[4] Vet Affairs Puget Sound Hlth Care Syst, Geriatr Res Educ & Clin Ctr, Seattle, WA USA

[5] Univ Washington, Sch Med, Dept Psychiat & Behav Sci, Seattle, WA 98104 USA

[6] Vet Affairs Puget Sound Hlth Care Syst, Mental Illness Res Educ & Clin Ctr, Seattle, WA USA

[7] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Neurosci & Behav, Sao Paulo, Brazil

[8] St Olavs Univ Hosp, Dept Neurol, Trondheim, Norway

关键词: Parkinson's disease;    Neurodegeneration;    Movement disorder;    alpha-Synuclein;    DJ-1;    Saliva;    Biomarker;   

DOI  :  10.1016/j.neurobiolaging.2013.08.008

来源: Elsevier

【 摘 要 】

Recently, alpha-synuclein (alpha-syn) and DJ-1, 2 proteins critically involved in Parkinson's disease (PD), have been shown to be present in saliva, suggesting their potential utility as biomarkers of PD. However, the origin and influence of demographic characteristics (e.g., age or sex) on these proteins are unknown. We identified cheek epithelium, which forms the majority of the cellular component of saliva and is readily accessible clinically, as 1 of several potential sources of salivary alpha-syn and DJ-1. However, no PD-related trend in the cellular component was present. In the supernatant collected from 198 healthy subjects, no correlation was seen between salivary DJ-1 or alpha-syn with age. When male and female subjects were analyzed separately, a weak age-dependent increase in DJ-1 level was present in male subjects, along with slightly increased alpha-syn in female subjects. These results, albeit largely negative, provide critical information for understanding the salivary gland pathology and saliva as a PD biomarker source, and must be considered in future investigations of salivary changes in PD. (C) 2014 Elsevier Inc. All rights reserved.

【 授权许可】

Free