Amyloid β1-42 oligomer inhibits myelin sheet formation in vitro

NEUROBIOLOGY OF AGING

卷:33

Article

Horiuchi, Makoto^1,2  Maezawa, Izumi^3,4  Itoh, Aki^1,2  Wakayama, Kouji^1,2  Jin, Lee-Way^3,4  Itoh, Takayuki^1,2  DeCarli, Charles¹ 

[1] Univ Calif Davis, Dept Neurol, Sch Med, Sacramento, CA 95817 USA

[2] Shriners Hosp Children No Calif, Inst Pediat Regenerat Med, Sacramento, CA USA

[3] Univ Calif Davis, MIND Inst, Ctr Canc, Sacramento, CA 95817 USA

[4] Univ Calif Davis, Dept Pathol, Dept Internal Med, Ctr Canc, Sacramento, CA 95817 USA

关键词: Alzheimer's disease;    Amyloid beta;    Lipid;    Myelin;    Oligodendroglia;    White matter degeneration;   

DOI  :  10.1016/j.neurobiolaging.2010.05.007

来源: Elsevier

【 摘 要 】

Accumulating evidence indicates that white matter degeneration contributes to the neural disconnections that underlie Alzheimer's disease pathophysiology. Although this white matter degeneration is partly attributable to axonopathy associated with neuronal degeneration, amyloid beta (A beta) protein-mediated damage to oligodendrocytes could be another mechanism. To test this hypothesis, we studied effects of soluble Lambda beta in oligomeric form on survival and differentiation of cells of the oligodendroglial lineage using highly purified oligodendroglial cultures from rats at different developmental stages. A beta oligomer at 10 mu M or higher reduced survival of mature oligodendrocytes, whereas oligodendroglial progenitor cells (OPCs) were relatively resistant to the A beta oligomer-mediated cytotoxicity. Further study revealed that A beta oligomer even at 1 mu M accelerated 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) formazan exocytosis in mature oligodendrocytes, and, more significantly, inhibited myelin sheet formation after induction of in vitro differentiation of OPCs. These results imply a novel pathogenetic mechanism underlying A beta oligomer-mediated white matter degeneration, which could impair myelin maintenance and remyelination by adult OPCs, resulting in accumulating damage to myelinating axons thereby contributing to neural disconnections. (C) 2012 Elsevier Inc. All rights reserved.

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