The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality

REPRODUCTIVE BIOMEDICINE ONLINE

卷:30

Article

Valckx, Sara D. M.¹  De Bie, Jessie¹  Michiels, Ellen D.¹  Goovaerts, Ilse G.²  Punjabi, Usha²  Ramos-Ibeas, Priscila³  Gutierrez-Adan, Alfonso³  Bols, Peter E.¹  Leroy, Jo L.¹ 

[1] Univ Antwerp, Dept Vet Sci, B-2610 Antwerp, Belgium

[2] Univ Antwerp Hosp, Ctr Reprod Med, B-2650 Edegem, Belgium

[3] Inst Nacl Invest & Tecnol Agr & Alimentaria, INIA, Dept Reprod Anim, Madrid 28040, Spain

关键词: embryo quality;    follicular fluid;    in-vitro maturation;    IVF;    oocyte developmental competence;   

DOI  :  10.1016/j.rbmo.2014.10.008

来源: Elsevier

【 摘 要 】

In this study, the hypothesis that embryo development during routine IVF procedures is determined by the pre-ovulatory follicular fluid composition was tested. Follicular fluid from women with obesity ('obese') and a 'positive' or ` negative' IVF outcome was added during the in-vitro maturation of bovine oocytes. 'Negative' and 'obese' follicular fluid reduced bovine embryo development, compared with laboratory control embryo development (P < 0.05 or P < 0.1). The addition of follicular fluid also altered bovine blastocyst gene expression. Furthermore, LDHA and PPARGC1B gene expression differed between follicular fluid groups. Data suggest that pre-ovulatory follicular fluid can potentially affect oocyte developmental competence and embryo quality. Furthermore, the bovine model may be used as a screening tool. (C) 2014 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

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