Does supplementation of in-vitro culture medium with melatonin improve IVF outcome in PCOS?

REPRODUCTIVE BIOMEDICINE ONLINE

卷:26

Article

Kim, Mi Kyoung¹  Park, Eun A.¹  Kim, Hyung Joon¹  Choi, Won Yun¹  Cho, Jung Hyun¹  Lee, Woo Sik¹  Cha, Kwang Yul¹  Kim, You Shin¹  Lee, Dong Ryul¹  Yoon, Tae Ki¹ 

[1] CHA Univ, Coll Med, CHA Gangnam Med Ctr, Fertil Ctr, Seoul 135081, South Korea

关键词: culture media;    in-vitro maturation;    IVF;    melatonin;    oocyte maturation;    PCOS;   

DOI  :  10.1016/j.rbmo.2012.10.007

来源: Elsevier

【 摘 要 】

Human pre-ovulatory follicular fluid (FF) contains a higher concentration of melatonin than serum. The aim of this study was to evaluate the effect of melatonin supplementation of culture medium on the clinical outcomes of an in-vitro maturation (IVM) IVF-embryo transfer programme for patients with polycystic ovarian syndrome (PCOS). Melatonin concentrations in the culture media of granulosa cells (GC) or cumulus-oocyte-complexes (COC) were measured and the clinical outcomes after using IVM media with or without melatonin were analysed. In the culture media of GC or COC, melatonin concentrations gradually increased. When human chorionic gonadotrophin priming protocols were used, implantation rates in the melatonin-supplemented group were higher than those of the non-supplemented control group (P < 0.05). Pregnancy rates were also higher, although not significantly. The findings suggest that the addition of melatonin to IVM media may improve the cytoplasmic maturation of human immature oocytes and subsequent clinical outcomes. It is speculated that follicular melatonin may be released from luteinizing GC during late folliculo-genesis and that melatonin supplementation may be used to improve the clinical outcomes of IVM IVF-embryo transfer. RBMOnline (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

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