Impact of meiotic and mitotic non-disjunction on generation of human embryonic stem cell lines

REPRODUCTIVE BIOMEDICINE ONLINE

卷:18

Article

Verlinsky, Yury¹  Strelchenko, Nikolai¹  Kukharenko, Valeri¹  Zech, Nicolas H.^1,2  Shkumatov, Artem¹  Zlatopolsky, Zev¹  Kuliev, Anver¹ 

[1] Illinois Masonic Med Ctr, Inst Reprod Genet, Chicago, IL 60657 USA

[2] Univ Zurich Hosp, Dept Obstet, Zurich, Switzerland

关键词: aneuploidy rescue;    human embryonic stem cells;    in-vitro fertilization;    non-disjunction;    polar body diagnosis;    preimplantation genetic diagnosis;   

DOI  :  10.1016/S1472-6483(10)60433-6

来源: Elsevier

【 摘 要 】

At least 50-60% of oocytes derived from IVF procedures are chromosomally abnormal due to meiotic I or II errors. Through the use of polar body and blastomere diagnosis, euploid embryos Suitable for transfer can be identified. Those embryos that are aneuploid are usually discarded, or otherwise can be used to generate chromosomally abnormal human embryonic stem cell (hESC) lines. The authors' centre has one of the largest repositories of hESC lilies with genetic and chromosomal disorders generated from preimplantation genetic diagnosis (PGD) abnormal embryos. The results, studying hESC lilies derived from PGD abnormal zygotes, imply that aneuploidies resulting from meiotic non-disjunction have a greater impact on viability of cells of the human embryos than those originating from post-zygotic mitotic non-disjunction.

【 授权许可】

Free