期刊论文详细信息
REPRODUCTIVE BIOMEDICINE ONLINE 卷:34
Embryos with morphokinetic abnormalities may develop into euploid blastocysts
Article
Lagalla, C.1  Tarozzi, N.1  Sciajno, R.1  Wells, D.2,3  Di Santo, M.1  Nadalini, M.1  Distratis, V.1  Borini, A.1 
[1] 9 Baby Ctr Reprod Hlth, Via Dante 15, I-40125 Bologna, Italy
[2] Inst Reprod Sci, Reprogenet UK, Business Pk North, Oxford 0X4 2HW, England
[3] Univ Oxford, Nuffield Dept Obstet & Gynaecol, Oxford OX3 9DU, England
关键词: aneuploidy rescue;    arrayCGH;    irregular cell divisions;    morphokinetics;    morula compaction;    pre-implantation genetic screening;    time-lapse;   
DOI  :  10.1016/j.rbmo.2016.11.008
来源: Elsevier
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【 摘 要 】

Irregular cleavage divisions are expected to produce chromosomally deviant embryos. We investigated whether embryos from irregular cleavages could develop into euploid blastocysts, and, if so, whether any evidence existed of a self-correction mechanism of the embryo. We also investigated the role of different dynamic aspects of morula compaction in this process. A total of 791 embryos from 141 patients undergoing pre-implantation genetic screening were retrospectively analysed using a time-lapse imaging system, and multiple cell divisions were evaluated. A total of 276 embryos developed into blastocysts suitable for biopsy and chromosome screening through array-comparative genomic hybridization. As well as testing trophectoderm biopsy specimens for aneuploidy, excluded cells of 18 blastocysts, which developed from partially compacted morulas, were also analysed. Unique data on the developmental fate of embryos with cleavage abnormalities are presented, and a potential mechanism of 'aneuploidy rescue' is postulated through which mosaic embryos may form partially compacted morulas to exclude aneuploid cells. In addition, this process seems to be less efficient in older women. The data obtained also provide further evidence that excluded cells should not be used to infer the cytogenetic status of the embryo. (C) 2016 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

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