Impact of sperm DNA fragmentation on the outcome of IVF with own or donated oocytes

REPRODUCTIVE BIOMEDICINE ONLINE

卷:23

Article

Esbert, Marga¹  Pacheco, Alberto²  Vidal, Francesca³  Florensa, Mireia¹  Riqueros, Marissa¹  Ballesteros, Agustin¹  Garrido, Nicolas⁴  Calderon, Gloria¹ 

[1] IVI Barcelona, IVF Lab, Barcelona 08017, Spain

[2] IVI Madrid, Madrid, Spain

[3] Univ Autonoma Barcelona, E-08193 Barcelona, Spain

[4] IVI Valencia, Valencia, Spain

关键词: DNA fragmentation;    embryo quality;    IVF;    miscarriage;    oocyte donors;   

DOI  :  10.1016/j.rbmo.2011.07.010

来源: Elsevier

【 摘 要 】

A prospective study was performed to assess the impact of sperm DNA fragmentation on the outcome of IVF with own or donated oocytes. The study population included 178 couples (62 cycles of IVF, 116 of intracytoplasmic sperm injection (ICSI)) with own (n = 77) and donor (n = 101) oocytes. DNA fragmentation was evaluated by TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling assay. Correlation between DNA damage to oocyte fertilization, embryo quality and clinical pregnancy, implantation and miscarriage rates was evaluated. DNA fragmentation was not related to fertilization rates in either IVF (r = 0.08) or ICSI (r = -0.04) cycles. DNA fragmentation was similar in patients with <50% embryo utilization rate compared with >= 50%, in cancelled and in embryo transfer cycles and in miscarriages and in successful deliveries. Moreover, DNA fragmentation was similar in pregnant and non-pregnant women as well as in IVF with own or donor oocytes. In the multivariable analysis, the odds ratio of DNA after controlling by age was 1.0. Using a 36% sperm fragmentation threshold, results did not vary. It is concluded that DNA damage was not related to outcomes of IVF or ICSI with own or donor oocytes. (C) 2011, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

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