期刊论文详细信息
RENEWABLE ENERGY 卷:105
Process optimization for biodiesel production from waste cooking oil using multi-enzyme systems through response surface methodology
Article
Babaki, Mohadese1  Yousefi, Maryam2  Habibi, Zohreh1  Mohammadi, Mehdi3 
[1] Shahid Beheshti Univ, GC, Fac Chem, Dept Pure Chem, Tehran, Iran
[2] ACECR, Avicenna Res Inst, Nanobiotechnol Res Ctr, Tehran, Iran
[3] NIGEB, Inst Ind & Environm Biotechnol, Bioproc Engn Dept, Tehran, Iran
关键词: Biodiesel;    Lipase;    Immobilization;    Waste cooking oil;    Response surface methodology;   
DOI  :  10.1016/j.renene.2016.12.086
来源: Elsevier
PDF
【 摘 要 】

Lipase from Rhizomucor miehei (RML) and lipase B from Candida antarctica (CALB) were covalently immobilized onto epoxy-functionalized silica. In this study, we developed a multi-enzyme system to produce biodiesel with waste cooking oil and methanol. To increase the biodiesel production yield, a mixture of 1,3-specific lipase (RML) and nonspecific lipase (CALB) was used. Response Surface Methodology (RSM) and a central composite rotatable design (CCRD) was used to study the effects of four factors, CALB:RML ratio, ratio of t-butanol to oil (wt.%), water adsorbent Content (wt.%) and reaction time on the fatty acid methyl esters (FAME) yield. A quadratic polynomial equation was obtained for methanolysis reaction by multiple regression analysis. The optimum combinations for the reaction were CALB:RML ratio (3:1), t-butanol to oil (10 wt%), water adsorbent content (22.5 wt%) at the reaction time of 10 h. FAME yield of 91.5%, which was very close to the predicted value of 95.6%, was obtained. Verification experiment confirmed the validity of the predicted model. (C) 2017 Elsevier Ltd. All rights reserved.

【 授权许可】

Free   

【 预 览 】
附件列表
Files Size Format View
10_1016_j_renene_2016_12_086.pdf 653KB PDF download
  文献评价指标  
  下载次数:0次 浏览次数:0次