Blinded search for varicella zoster virus in giant cell arteritis (GCA)-positive and GCA-negative temporal arteries

JOURNAL OF THE NEUROLOGICAL SCIENCES

卷:364

Article

Gilden, Don^1,2  White, Teresa¹  Khmeleva, Nelly¹  Katz, Bradley J.^3,4  Nagel, Maria A.¹ 

[1] Univ Colorado, Sch Med, Dept Neurol, Aurora, CO USA

[2] Univ Colorado, Sch Med, Dept Immunol & Microbiol, Aurora, CO USA

[3] Univ Utah, Hlth Sci Ctr, Dept Ophthalmol & Visual Sci, Salt Lake City, UT USA

[4] Univ Utah, Hlth Sci Ctr, Dept Neurol, Salt Lake City, UT USA

关键词: Varicella zoster virus;    Giant cell arteritis;   

DOI  :  10.1016/j.jns.2016.03.020

来源: Elsevier

【 摘 要 】

Recent analysis of archived temporal arteries (TAs) acquired from 13 pathology laboratories in the US, Canada, Iceland, France, Germany and Israel from patients with pathologically-verified giant cell arteritis (GCA-positive) and TAs from patients with clinical features and laboratory abnormalities of GCA but whose TAs were pathologically negative (GCA-negative) revealed VZV antigen in most TAs from both groups. Despite formalin-fixation, VZV DNA was also found in many VZV-antigen positive sections that were scraped, subjected to DNA extraction, and examined by PCR with VZV-specific primers. Importantly, in past studies, the pathological diagnosis (GCA-positive or-negative) was known to the neurovirology laboratory. Herein, GCA-positive and GCA-negative TAs were provided by an outside institution and examined by 4 investigators blinded to the pathological diagnoses. VZV antigen was found in 3/3 GCA-positive TAs and in 4/6 GCA-negative TAs, and VZV DNA in 1/3 VZV antigen-positive, GCA-positive TAs and in 3/4 VZV antigen-positive, GCA-negative TAs. VZV DNA was also detected in one GCA-negative, VZV-antigen negative TA. Overall, the detection of VZV antigen in 78% of GCA-positive and GCA-negative TAs is consistent with previous reports on the prevalence of VZV antigen in patients with clinically suspect GCA. (C) 2016 Elsevier B.V. All rights reserved.

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