Conformational analysis of a covalently cross-linked Watson-Crick base pair model

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:18

Article

Jensen, Erik A.¹  Allen, Benjamin D.¹  Kishi, Yoshito²  O'Leary, Daniel J.¹ 

[1] Pomona Coll, Dept Chem, Claremont, CA 91711 USA

[2] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

关键词: Nucleic acids;    DNA;    Covalent cross-linked base pair;    Watson-Crick;    Dynamic NMR;   

DOI  :  10.1016/j.bmcl.2008.07.113

来源: Elsevier

【 摘 要 】

Low-temperature NMR experiments and molecular modeling have been used to characterize the conformational behavior of a covalently cross-linked DNA base pair model. The data suggest that Watson-Crick or reverse Watson-Crick hydrogen bonding geometries have similar energies and can interconvert at low temperatures. This low-temperature process involves rotation about the crosslink CH(2)AC(5') (psi) carbon carbon bond, which is energetically preferred over the alternate CH2-N(3) (phi) carbon-nitrogen bond rotation. (C) 2008 Elsevier Ltd. All rights reserved.

【 授权许可】

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