Synthesis and properties of triazole-linked RNA

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:21

Article

Mutisya, Daniel¹  Selvam, Chelliah¹  Kennedy, Scott D.²  Rozners, Eriks¹ 

[1] SUNY Binghamton, Dept Chem, Binghamton, NY 13902 USA

[2] Univ Rochester, Sch Med & Dent, Dept Biochem & Biophys, Rochester, NY 14642 USA

关键词: RNA;    Modified nucleoside;    Triazole internucleoside linkage;    Huisgen dipolar cycloaddition;    Click reaction;   

DOI  :  10.1016/j.bmcl.2011.03.111

来源: Elsevier

【 摘 要 】

RNA oligonucleotides having triazole linkages between uridine and adenosine nucleosides have been prepared and studied using spectroscopic techniques. UV melting and CD showed that triazole strongly destabilized RNA duplex (7-14 degrees C per modification). NMR data suggested that, despite relative flexibility around the modified linkage, all base pairs were formed. (C) 2011 Elsevier Ltd. All rights reserved.

【 授权许可】

Free