Quantitative determination of linking number differences between circular polynucleosomes and histone H1-bound circular polynucleosomes

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:28

Article

Zhang, Hao¹  Li, Tianhu¹ 

[1] Nanyang Technol Univ, Sch Phys & Math Sci, Div Chem & Biol Chem, 21 Nanyang Link, Singapore 637371, Singapore

关键词: Nucleosome;    Histone octamer;    Histone H1;    Linking number;    Polynucleosome;   

DOI  :  10.1016/j.bmcl.2017.10.072

来源: Elsevier

【 摘 要 】

With the aim of discovering contribution of histone H1 to linking number changes of DNA, determination of linking number differences between histone H1-free circular polynucleosomes and histone H1-bound circular polynucleosomes was carried out during our investigations. Our results showed that on average, binding of similar to 11.5 histone H1 molecules causes one linking number change in circular polynucleosomes in the presence of 1.5 mM spermidine. When concentrations of spermidine decreases or increases, these linking number differences decrease significantly. It is therefore evident that linking number changes caused by histone H1 are spermidine concentration-dependent. (C) 2017 Elsevier Ltd. All rights reserved.

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