Folding of single‐stranded DNA on the histone octamer

FEBS Letters

Caffarelli, E.¹  Leoni, L.¹  Savino, M.¹ 

[1] Centra di Studio per gli Acidi Nucleici, Dipartimento di Genetica e Biologia Molecolare, Università degli Studi di Roma I, Roma, Italy

关键词: Chromatin;    Single-stranded DNA;    Histone octamer;    Electron microscopy;    Low-angle X-ray diffraction;    Nuclease analysis;   

DOI  :  10.1016/0014-5793(85)81115-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A complex between the single-stranded DNA of the bacteriophage M13 and the histone octamer was analyzed by electron microscopy, low-angle X-ray diffraction and nuclease analysis. The morphology and the diffraction pattern of the complex strongly resemble those of the nucleosome. These results, as well as the finding of a protected DNA fragment about 100 nucleotides long following single-stranded DNA specific nuclease digestion, indicate that ‘a nucleosome-like’ complex can be formed between single-stranded DNA and the histone octamer. Competition experiments suggest that under physiological conditions the histone octamer is transferred from single- to double-stranded DNA.

【 授权许可】

Unknown