Effects of Different Buffers on the Construction of Aptamer Sensors

1st International Conference on Frontiers of Materials Synthesis and Processing

材料科学;化学

Yu, Quan^1 ; Dai, Zhao^1 ; Wu, Wenjing^1 ; Zhu, Haijia^1 ; Ji, Luyu^1

School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin

300387, China^1

关键词: Aptamer sensor;    Carboxyfluorescein;    Detection ability;    Detection process;    Donor and acceptor;    Energy donors;    Fluorescence energy resonance transfers;    Single-stranded DNA;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/274/1/012117/pdf
DOI  :  10.1088/1757-899X/274/1/012117

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

In this paper, the effect of different buffers, PBS and TBE, on the construction of an aptamer sensor (apt sensor) for ATP was investigated. The apt sensor was based on fluorescence energy resonance transfer (FRET), when the energy donor was 5'-carboxyfluorescein (5'-FAM) and the energy receptor was Au nanoparticles (AuNPs), respectively. Both the donor and acceptor were conjugated with complementary and single stranded DNA (ssDNA). The fluorescent changes of the sensors were measured to investigate the influence of different buffers during the whole preparation and detection process. The results indicated that when the AuNPs and ssDNA (Au-DNA1) were conjugated in PBS buffer, the corresponding apt sensors would obtain a better detection ability of ATP than in TBE buffer.