Carbon nanodots as molecular scaffolds for development of antimicrobial agents

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:26

Article

Ngu-Schwemlein, Maria¹  Chin, Suk Fun²  Hileman, Ryan¹  Drozdowski, Chris¹  Upchurch, Clint¹  Hargrove, April¹ 

[1] Winston Salem State Univ, Dept Chem, Winston Salem, NC 27110 USA

[2] Univ Malaysia Sarawak, Dept Chem, Kota Samarahan 94300, Sarawak, Malaysia

关键词: Carbon nanodots;    PAMAM;    Molecular scaffold;    Antimicrobial activities;    Synergism;   

DOI  :  10.1016/j.bmcl.2016.02.047

来源: Elsevier

【 摘 要 】

We report the potential of carbon nanodots (CNDs) as a molecular scaffold for enhancing the antimicrobial activities of small dendritic poly(amidoamines) (PAMAM). Carbon nanodots prepared from sago starch are readily functionalized with PAMAM by using N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Electron microscopy images of these polyaminated CNDs show that they are approximately 30-60 nm in diameter. Infrared and fluorescence spectroscopy analyses of the water-soluble material established the presence of the polyamidoaminated moiety and the intrinsic fluorescence of the nanodots. The polyaminated nanodots (CND-PAM1 and CND-PAM2) exhibit in vitro antimicrobial properties, not only to non-multidrug resistant bacteria but also to the corresponding Gram-negative multidrug bacteria. Their minimum inhibitory concentration (MIC) ranges from 8 to 64 mu g/mL, which is much lower than that of PAMAM G1 or the non-active PAMAM G0 and CNDs. Additionally, they show synergistic effect in combination with tetracycline or colistin. These preliminary results imply that CNDs can serve as a promising scaffold for facilitating the rational design of antimicrobial materials for combating the ever-increasing threat of antibiotic resistance. Moreover, their fluorescence could be pertinent to unraveling their mode of action for imaging or diagnostic applications. (C) 2016 Elsevier Ltd. All rights reserved.

【 授权许可】

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