Fluorescence detection of serum albumin with a turnover-based sensor utilizing Kemp elimination reaction

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

卷:27

Article

Sakamoto, Shingo¹  Komatsu, Toru¹  Ueno, Tasuku¹  Hanaoka, Kenjiro¹  Urano, Yasuteru^1,2,3 

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan

[2] Univ Tokyo, Grad Sch Med, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan

[3] Japan Agcy Med Res & Dev AMED, CREST, Chiyoda Ku, 1-7-1 Otemachi, Tokyo 1000004, Japan

关键词: Chemical biology;    Fluorescent probes;    Proteins;    Enzymes;    Kemp elimination;   

DOI  :  10.1016/j.bmcl.2017.05.076

来源: Elsevier

【 摘 要 】

The Kemp elimination reaction is a well-known chemical reaction that is facilitated on a protein surface microenvironment, and in particular is highly accelerated in a unique binding pocket of serum albumin. We have designed and synthesized a fluorescently activatable coumarin derivative with a benzisoxazole scaffold to enable monitoring of the Kemp elimination reaction in terms of fluorescence change for the first time. We show that this fluorescent sensor can sensitively and selectively quantitate serum albumin in blood samples. It also works in a dry-chemistry format. (C) 2017 Elsevier Ltd. All rights reserved.

【 授权许可】

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