Influence of magnetic field on enzymatic ONOO_ production

3rd International Conference and Young Scientist School "Magnetic resonance imaging in biomedical research"

物理学;医药卫生

Dranova, T.^1 ; Petrovskii, D.^1,2 ; Ershov, N.^2 ; Slepneva, I.^1 ; Stass, D.^1

Voevodsky Institute of Chemical Kinetics and Combustion, Novosibirsk, Russia^1

Federal Research Center, Institute of Cytology and Genetics, Novosibirsk, Russia^2

关键词: Biochemical process;    Enzymatic oxidations;    Fluorescent probes;    Inducible nitric oxide synthase;    Influence of magnetic field;    Peroxynitrites;    Reactive species;    Superoxide anions;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/886/1/012004/pdf
DOI  :  10.1088/1742-6596/886/1/012004

学科分类：卫生学

来源: IOP

【 摘 要 】

Enzymatic oxidation of L-arginine catalyzed by inducible nitric oxide synthase gives nitric oxide as the main product and superoxide anion as a side reaction product. Recombination of these radicals gives a very reactive species - peroxynitrite, which is involved in many biochemical processes. In the current work it was shown that such a system can be a usable model system for investigating the influence of magnetic field on enzymatic peroxynitrite formation. Using a selective fluorescent probe for peroxynitrite - coumarin boronic acid and an adopted for the experimental purpose incubation mixture, magnetic field experiments have been done at 11.7T. The averaged magnetic field effect is equal to 2.8±0.9%.