Impaired fear response in mice lacking GIT1

NEUROSCIENCE LETTERS

卷:458

Article

Schmalzigaug, Robert¹  Rodriguiz, Ramona M.^2,5  Bonner, Pamela E.¹  Davidson, Collin E.¹  Wetsel, William C.^2,3,4,5  Premont, Richard T.¹ 

[1] Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA

[2] Duke Univ, Med Ctr, Dept Psychiat & Behav Sci, Durham, NC 27710 USA

[3] Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA

[4] Duke Univ, Med Ctr, Dept Neurobiol, Durham, NC 27710 USA

[5] Duke Univ, Med Ctr, Mouse Behav & Neuroendocrine Anal Core Facil, Durham, NC 27710 USA

关键词: GIT1;    GIT2;    Knockout mice;    Behavior;    Fear conditioning;   

DOI  :  10.1016/j.neulet.2009.04.037

来源: Elsevier

【 摘 要 】

G protein-coupled receptor kinase interacting protein 1 (GIT1) belongs to the family of Arf GAP proteins and has been implicated in the regulation of G protein-coupled receptor (GPCR) sequestration, cell migration, synapse formation and dendritic spine morphogenesis in neurons. To extend these cellular studies on GIT1 to an in vivo system, we generated mice with globally inactivated Git1 gene by breeding mice carrying a conditional Git1(flox) allele with mice expressing the CMV-Cre transgene. Although many GIT1 knockout (GIT1-KO) animals died shortly after birth, homozygous mutants that survived the early postpartum period developed normally into adulthood and were fertile. Behavioral analyses of adult GIT1-KO mice revealed normal exploratory, anxiety- and depressive-like behaviors. However, GIT1-KO mice show impaired responses to fear conditioning and fear-potentiated startle. Overall, these findings suggest that GIT1 is involved in the regulation of amygdala-mediated experience-based emotional behaviors. (c) 2009 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

Free