MR histology of advanced atherosclerotic lesions of ApoE- knockout mice

2nd International Conference and Young Scientist School "Magnetic resonance imaging in biomedical research"

Naumova, A.^1,2 ; Yarnykh, V.^1,2 ; Ferguson, M.^1 ; Rosenfeld, M.^3 ; Yuan, C.^4

Vascular Imaging Lab, Department of Radiology, University of Washington, Seattle

WA, United States^1

Neurobiology Lab, Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, Russia^2

Department of Pathobiology, University of Washington, Seattle, United States^3

Departments of Radiology and Bioengineering, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle

WA, United States^4

关键词: Atherosclerotic lesions;    Atherosclerotic plaque;    Carotid artery;    Correlation coefficient;    Field of views;    Gradient echo sequences;    Knockout mice;    Time-efficient;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/677/1/012008/pdf
DOI  :  10.1088/1742-6596/677/1/012008

来源: IOP

【 摘 要 】

The purposes of this study were to examine the feasibility of determining the composition of advanced atherosclerotic plaques in fixed ApoE-knockout mice and to develop a time-efficient microimaging protocol for MR histological imaging on mice. Five formalin-fixed transgenic ApoE-knockout mice were imaged at the 9.4T Bruker BioSpec MR scanner using 3D spoiled gradient-echo sequence with an isotropic field of view of 24 mm3; TR 20.8 ms; TE 2.6 ms; flip angle 20°, resulted voxel size 47 × 63 × 94 pm3. MRI examination has shown that advanced atherosclerotic lesions of aorta, innominate and carotid arteries in ApoE-knockout mice are characterized by high calcification and presence of the large fibrofatty nodules. MRI quantification of atherosclerotic lesion components corresponded to histological assessment of plaque composition with a correlation coefficient of 0.98.