Neuronal-glial populations form functional networks in a biocompatible 3D scaffold

NEUROSCIENCE LETTERS

卷:609

Article

Smith, Imogen^1,4  Haag, Marcus¹  Ugbode, Christopher^1,2  Tams, Daniel³  Rattray, Marcus²  Przyborski, Stefan³  Bithell, Angela¹  Whalley, Benjamin J.¹ 

[1] Univ Reading, Sch Chem Food & Nutr Sci & Pharm, Reading RG6 6AP, Berks, England

[2] Univ Bradford, Sch Pharm, Bradford BD7 1DP, W Yorkshire, England

[3] Univ Durham, Sch Biol & Biomed Sci, Durham DH1 3LE, England

[4] Univ Portsmouth, Portsmouth Brain Tumour Res Ctr, Portsmouth PO1 2DT, Hants, England

关键词: Cortical cultures;    Microelectrode array;    Biocompatible scaffold;    3D Neuronal culture;    Murine;   

DOI  :  10.1016/j.neulet.2015.10.044

来源: Elsevier

【 摘 要 】

Monolayers of neurons and glia have been employed for decades as tools for the study of cellular physiology and as the basis for a variety of standard toxicological assays. A variety of three dimensional (3D) culture techniques have been developed with the aim to produce cultures that recapitulate desirable features of intact. In this study, we investigated the effect of preparing primary mouse mixed neuron and glial cultures in the inert 3D scaffold, Alvetex. Using planar multielectrode arrays, we compared the spontaneous bioelectrical activity exhibited by neuroglial networks grown in the scaffold with that seen in the same cells prepared as conventional monolayer cultures. Two dimensional (monolayer; 2D) cultures exhibited a significantly higher spike firing rate than that seen in 3D cultures although no difference was seen in total signal power (<50 Hz) while pharmacological responsiveness of each culture type to antagonism of GABA(A)R, NMDAR and AMPAR was highly comparable. Interestingly, correlation of burst events, spike firing and total signal power (<50 Hz) revealed that local field potential events were associated with action potential driven bursts as was the case for 2D cultures. Moreover, glial morphology was more physiologically normal in 3D cultures. These results show that 3D culture in inert scaffolds represents a more physiologically normal preparation which has advantages for physiological, pharmacological, toxicological and drug development studies, particularly given the extensive use of such preparations in high throughput and high content systems. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

Free