Efficient Delivery of Human Single Fiber-Derived Muscle Precursor Cells via Biocompatible Scaffold

Cell Transplantation

Article

Elisa Cimetta¹  Nicola Elvassore¹  Libero Vitiello²  Pier Giorgio Gamba³  Martina Piccoli⁴  Chiara Messina⁴  Alberto Malerba⁵  Luisa Boldrin⁵  Paolo De Coppi⁵ 

[1] Department of Chemical Engineering, University of Padova, Padova, Italy;Gene Transfer Laboratory, Department of Biology, University of Padova, Padova, Italy;Paediatric Surgery, Department of Paediatrics, University of Padova, Padova, Italy;Stem Cell Processing Laboratory, Department of Paediatrics, University of Padova, Padova, Italy;Stem Cell Processing Laboratory, Department of Paediatrics, University of Padova, Padova, Italy;Surgery Unit, Institute of Child Health, University College of London, London, UK;

关键词: Human single fibers;    Muscle precursor cells;    Biocompatible scaffold;    Muscle regeneration;   

DOI  :  10.3727/096368908785095980

 received in 2007-02-19, accepted in 2007-10-06,  发布年份 2008

来源: Sage Journals

【 摘 要 】

The success of cell therapy for skeletal muscle disorders depends upon two main factors: the cell source and the method of delivery. In this work we have explored the therapeutic potential of human muscle precursor cells (hMPCs), obtained from single human muscle fibers, implanted in vivo via micropatterned scaffolds. hMPCs were initially expanded and characterized in vitro by immunostaining and flow cytometric analysis. For in vivo studies, hMPCs were seeded onto micropatterned poly-lactic-glycolic acid 3D-scaffolds fabricated using soft-lithography and thermal membrane lamination. Seeded scaffolds were then implanted in predamaged tibialis anterior muscles of CD1 nude mice; hMPCs were also directly injected in contralateral limbs as controls. Similarly to what we previously described with mouse precursors cells, we found that hMPCs were able to participate in muscle regeneration and scaffold-implanted muscles contained a greater number of human nuclei, as revealed by immunostaining and Western blot analyses. These results indicate that hMPCs derived from single fibers could be a good and reliable cell source for the design of therapeutic protocols and that implantation of cellularized scaffolds is superior to direct injection for the delivery of myogenic cells into regenerating skeletal muscle.

【 授权许可】

Unknown   
© 2008 Cognizant Comm. Corp.