Effect of prolonged differentiation on functional maturation of human pluripotent stem cell-derived neuronal cultures - ScienceDirect

Stem Cell Research

Tanja Paavilainen¹ 

关键词: Astrocytes;    Calcium signaling;    Microelectrode array;    Neural development;    Neurons;    Pluripotent stem cells;   

DOI  :  10.1016/j.scr.2018.01.018

学科分类：生物科学（综合）

来源: Elsevier

【 摘 要 】

Long-term neural differentiation of human pluripotent stem cells (hPSCs) is associated with enhanced neuronal maturation, which is a necessity for creation of representative in vitro models. It also induces neurogenic-to-gliogenic fate switch, increasing proportion of endogenous astrocytes formed from the common neural progenitors. However, the significance of prolonged differentiation on the neural cell type composition and functional development of hPSC-derived neuronal cells has not been well characterized. Here, we studied two hPSC lines, both of which initially showed good neuronal differentiation capacity. However, the propensity for endogenous astrogenesis and maturation state after extended differentiation varied. Live cell calcium imaging revealed that prolonged differentiation facilitated maturation of GABAergic signaling. According to extracellular recordings with microelectrode array (MEA), neuronal activity was limited to fewer areas of the culture, which expressed more frequent burst activity. Efficient maturation after prolonged differentiation also promoted organization of spontaneous activity by burst compaction. These results suggest that although prolonged neural differentiation can be challenging, it has beneficial effect on functional maturation, which can also improve transition to different neural in vitro models and applications.

【 授权许可】

Unknown