Fibrin conduit supplemented with human mesenchymal stem cells and immunosuppressive treatment enhances regeneration after peripheral nerve injury

NEUROSCIENCE LETTERS

卷:516

Article

McGrath, Aleksandra M.^1,2  Brohlin, Maria¹  Kingham, Paul J.¹  Novikov, Lev N.¹  Wiberg, Mikael^1,2  Novikova, Liudmila N.¹ 

[1] Umea Univ, Sect Anat, Dept Integrat Med Biol, S-90187 Umea, Sweden

[2] Umea Univ, Sect Hand & Plast Surg, Dept Surg & Perioperat Sci, S-90187 Umea, Sweden

关键词: Peripheral nerve injury;    Nerve conduit;    Bone marrow;    Mesenchymal stem cells;    Regeneration;   

DOI  :  10.1016/j.neulet.2012.03.041

来源: Elsevier

【 摘 要 】

To address the need for the development of bioengineered replacement of a nerve graft, a novel two component fibrin glue conduit was combined with human mesenchymal stem cells (MSC) and immuno-supressive treatment with cyclosporine A. The effects of MSC on axonal regeneration in the conduit and reaction of activated macrophages were investigated using sciatic nerve injury model. A 10 mm gap in the sciatic nerve of a rat was created and repaired either with fibrin glue conduit containing diluted fibrin matrix or fibrin glue conduit containing fibrin matrix with MSC at concentration of 80 x 10(6) cells/ml. Cells were labeled with PKH26 prior to transplantation. The animals received daily injections of cyclosporine A. After 3 weeks the distance of regeneration and area occupied by regenerating axons and ED1 positives macrophages was measured. MSC survived in the conduit and enhanced axonal regeneration only when transplantation was combined with cyclosporine A treatment. Moreover, addition of cyclosporine A to the conduits with transplanted MSC significantly reduced the ED1 macrophage reaction. (c) 2012 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

Free