Bone marrow vs Wharton’s jelly mesenchymal stem cells in experimental sepsis: a comparative study

Stem Cell Research & Therapy

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[1] 0000 0001 2194 6418, grid.29172.3f, Université de Lorraine, 54000, Nancy, France;0000 0000 8775 4825, grid.452436.2, Service de Biopathologie - Unité de Biologie des Tumeurs, Institut de Cancérologie de Lorraine, 54500, Vandœuvre-lès-Nancy, France;0000 0001 2194 6418, grid.29172.3f, Université de Lorraine, 54000, Nancy, France;EA 7300 Stress Immunité Pathogènes, 54500, vandoeuvre-lès-Nancy, France;0000 0004 1765 1301, grid.410527.5, CHRU de Nancy, Unité de Thérapie Cellulaire et banque de Tissus, Allée du Morvan, 54500, Vandoeuvre-lès-Nancy, France;0000 0001 2112 9282, grid.4444.0, CNRS, UMR 7365, 54500, Vandoeuvre-lès-Nancy, France;0000 0001 2194 6418, grid.29172.3f, Université de Lorraine, 54000, Nancy, France;0000 0004 1765 1301, grid.410527.5, CHRU de Nancy, Unité de Thérapie Cellulaire et banque de Tissus, Allée du Morvan, 54500, Vandoeuvre-lès-Nancy, France;INSERM UMRS-1116, Vandoeuvre-lès-Nancy, France;0000 0001 2112 9282, grid.4444.0, CNRS, UMR 7365, 54500, Vandoeuvre-lès-Nancy, France;0000 0001 2194 6418, grid.29172.3f, Université de Lorraine, 54000, Nancy, France;0000 0004 1765 1301, grid.410527.5, CHRU de Nancy, laboratoire anatomie et cytologie pathologiques, 54500, Vandoeuvre-lès-Nancy, France;INOTREM, 54500, Vandoeuvre-lès-Nancy, France;INSERM UMRS-1116, Vandoeuvre-lès-Nancy, France;0000 0001 2194 6418, grid.29172.3f, Université de Lorraine, 54000, Nancy, France;0000 0004 1765 1301, grid.410527.5, CHRU de Nancy, Service de Réanimation Médicale, Hôpital Central, 54000, Nancy, France;INSERM UMRS-1116, Vandoeuvre-lès-Nancy, France;0000 0001 2194 6418, grid.29172.3f, Université de Lorraine, 54000, Nancy, France;INOTREM, 54500, Vandoeuvre-lès-Nancy, France;

关键词: Mesenchymal stem cells;    Tissue source;    Wharton’s jelly;    Bone marrow;    Sepsis;   

DOI  :  10.1186/s13287-019-1295-9

来源: publisher

【 摘 要 】

BackgroundThe use of mesenchymal stem cells (MSCs) is being extensively studied in clinical trials in the setting of various diseases including diabetes, stroke, and progressive multiple sclerosis. The unique immunomodulatory properties of MSCs also point them as a possible therapeutic tool during sepsis and septic shock, a devastating syndrome associated with 30–35% mortality. However, MSCs are not equal regarding their activity, depending on their tissue origin. Here, we aimed at comparing the in vivo properties of MSCs according to their tissue source (bone marrow (BM) versus Wharton’s jelly (WJ)) in a murine cecal ligation and puncture (CLP) model of sepsis that mimics a human peritonitis. We hypothesized that MSC properties may vary depending on their tissue source in the setting of sepsis.MethodsCLP, adult, male, C57BL/6 mice were randomized in 3 groups receiving respectively 0.25 × 106 BM-MSCs, 0.25 × 106 WJ-MSCs, or 150 μL phosphate-buffered saline (PBS) intravenously 24 h after the CLP procedure.ResultsWe observed that both types of MSCs regulated leukocyte trafficking and reduced organ dysfunction, while only WJ-MSCs were able to improve bacterial clearance and survival.ConclusionThis study highlights the importance to determine the most appropriate source of MSCs for a given therapeutic indication and suggests a better profile for WJ-MSCs during sepsis.

【 授权许可】

CC BY