Physical exercise increases the production of tyrosine hydroxylase and CDNF in the spinal cord of a Parkinson's disease mouse model

NEUROSCIENCE LETTERS

卷:760

Article

Barbosa da Silva, Wagner Antonio^1,3  Oliveira, Karla Ferreira^1,3  Vitorino, Louise Caroline^2,3  Romao, Luciana Ferreira²  Allodi, Silvana^1,2,3  Correa, Clynton Lourenco^3,4 

[1] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Programa Posgrad Ciencias Biol Fisiol, Av Carlos Chagas Filho 373, BR-21941902 Rio De Janeiro, RJ, Brazil

[2] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Programa Posgrad Ciencias Morfol, Av Carlos Chagas Filho 373, BR-21941902 Rio De Janeiro, RJ, Brazil

[3] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Neurobiol Comparat & Dev, Av Carlos Chagas Filho 373,Bloco G2-001, BR-21941902 Rio De Janeiro, RJ, Brazil

[4] Univ Fed Rio de Janeiro, Fac Fisioterapia, HUCFF, R Rodolpho Paulo Rocco 255, BR-21941590 Rio De Janeiro, RJ, Brazil

关键词: Parkinson's disease;    Cerebral dopamine neurotrophic factor;    Tyrosine hydroxylase;    Exercise;    Animal model;   

DOI  :  10.1016/j.neulet.2021.136089

来源: Elsevier

【 摘 要 】

Previous research advocates that exercise is a non-pharmacological therapy for Parkinson's disease (PD). However, few studies have investigated the effects of exercise on central nervous system structures other than the nigrostriatal pathway by using PD animal models. This study investigated the effects of exercise on tyrosine hydroxylase (TH)-and cerebral dopamine neurotrophic factor (CDNF)-containing spinal-cord neurons. Male Swiss mice were divided into 4 groups: sedentary control (SEDCONT), exercise control (EXERCONT), sedentary Parkinson (SEDPD), and exercise Parkinson (EXERPD). The PD groups were submitted to a surgical procedure for stereotaxic bilateral injection of 6-hydroxydopamine into the striatum. TH-and CDNF-containing spinal-cord neurons were evaluated in all groups, using immunohistochemistry and western-blotting. TH content in the ventral horn differed notably between the SEDPD and EXERPD groups. CDNF content was highest in the EXERPD group. SEDPD and EXERPD groups differed the most, as shown by immunohistochemistry and western-blotting. The EXERPD group showed the most intense labeling in immunohistochemistry compared to the SEDCONT and EXERCONT groups. Therefore, we showed here that exercise increased the content of both TH and CDNF in the spinal-cord neurons of a bilateral PD mouse model. We may assume that the spinal cord is affected in a PD model, and therefore this central nervous system region deserves more attention from researchers dealing with PD.

【 授权许可】

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