The contribution of age structure to cell population responses to targeted therapeutics

JOURNAL OF THEORETICAL BIOLOGY

卷:311

Article

Gabriel, Pierre¹  Garbett, Shawn P.²  Quaranta, Vito²  Tyson, Darren R.²  Webb, Glenn F.³ 

[1] Univ Paris 06, UMR LJLL 7598, F-75252 Paris 5, France

[2] Vanderbilt Univ, Dept Canc Biol, Nashville, TN 37232 USA

[3] Vanderbilt Univ, Dept Math, Nashville, TN 37240 USA

关键词: Cell cycle;    Intermitotic time;    Renewal equation;    Exponentially modified Gaussian;   

DOI  :  10.1016/j.jtbi.2012.07.001

来源: Elsevier

【 摘 要 】

Cells grown in culture act as a model system for analyzing the effects of anticancer compounds, which may affect cell behavior in a cell cycle position-dependent manner. Cell synchronization techniques have been generally employed to minimize the variation in cell cycle position. However, synchronization techniques are cumbersome and imprecise and the agents used to synchronize the cells potentially have other unknown effects on the cells. An alternative approach is to determine the age structure in the population and account for the cell cycle positional effects post hoc. Here we provide a formalism to use quantifiable lifespans from live cell microscopy experiments to parameterize an age-structured model of cell population response. (C) 2012 Elsevier Ltd. All rights reserved.,

【 授权许可】

Free