Metformin regulates mitochondrial biogenesis and senescence through AMPK mediated H3K79 methylation: Relevance in age-associated vascular dysfunction

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1864

Article

Karnewar, Santosh^1,2  Neeli, Praveen Kumar^1,2  Panuganti, Devayani¹  Kotagiri, Sasikumar¹  Mallappa, Sreevidya^1,2  Jain, Nishant^1,2  Jerald, Mahesh Kumar³  Kotamraju, Srigiridhar^1,2 

[1] CSIR Indian Inst Chem Technol, Ctr Chem Biol, Uppal Rd, Hyderabad 500007, Andhra Pradesh, India

[2] Acad Sci & Innovat Res, Training & Dev Complex, Madras 600113, Tamil Nadu, India

[3] CSIR Ctr Cellular & Mol Biol, Uppal Rd, Hyderabad 500007, Andhra Pradesh, India

关键词: Metformin;    Mitochondrial function;    Endothelial dysfunction;    Cardiovascular diseases;    Atherosclerosis;    Aging;   

DOI  :  10.1016/j.bbadis.2018.01.018

来源: Elsevier

【 摘 要 】

Endothelial senescence in conjunction with mitochondrial dysfunction orchestrates age-associated cardiovascular disorders. In this study we investigated the causal link between these two processes and studied the molecular mechanisms by which metformin acts to coordinate the delay of endothelial senescence via enhancing mitochondrial biogenesis/function. AMPK activators metformin and AICAR delayed endothelial senescence via SIRT1-mediated upregulation of DOT1L, leading to increased trimethylation of H3K79 (H3K79me3). Treatment of cells with either siAMPK or siSIRT1 repressed DOT1L-mediated enhancement of H3K79me3. Moreover, the increase in SIRT3 expression and mitochondrial biogenesis/function by AMPK activators was H3K79me-dependent as H3K79N mutant or siDOT1L abrogated these effects. This was confirmed by the enrichment of H3K79me3 in the SIRT3 promoter with AMPK activation. Intriguingly, enhanced PGC-1 alpha expression by SIRT3 via AMPK activation was responsible for increased hTERT expression and delayed endothelial senescence. In contrast, SIRT3 knockdown caused increased oxidative stress and premature senescence, possibly by depleting hTERT expression. Furthermore, a chronic low dose administration of metformin significantly attenuated vascular aging and inhibited age-associated atherosclerotic plaque formation in ApoE(-/-) mice. Overall, the results of this study show a novel regulation of mitochondrial biogenesis/function, and cellular senescence by H3K79me acting through SIRT3, thus providing a molecular basis for metformin-mediated age-delaying effects.

【 授权许可】

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