Proteostasis in epicardial versus subcutaneous adipose tissue in heart failure subjects with and without diabetes

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1864

Article

Burgeiro, A.¹  Fonseca, A. C.¹  Espinoza, D.¹  Carvalho, L.²  Lourenco, N.³  Antunes, M.⁴  Carvalho, E.^1,5,6,7 

[1] Univ Coimbra, Ctr Neurosci & Cell Biol, P-3004504 Coimbra, Portugal

[2] Univ Coimbra, Inst Pathol, Fac Med, P-3004517 Coimbra, Portugal

[3] Univ Coimbra, Ctr Informat & Syst, Dept Informat Engn, Coimbra, Portugal

[4] Coimbra Univ Hosp Ctr, Cardiothorac Surg Unit, P-3000075 Coimbra, Portugal

[5] Portuguese Diabet Assoc APDP, P-1250203 Lisbon, Portugal

[6] Univ Arkansas Med Sci, Dept Geriatr, Little Rock, AR 72202 USA

[7] Arkansas Childrens Res Inst, Little Rock, AR 72202 USA

关键词: Epicardial adipose tissue;    Endoplasmic reticulum stress;    Autophagy;    Apoptosis;    Heart failure;   

DOI  :  10.1016/j.bbadis.2018.03.025

来源: Elsevier

【 摘 要 】

Background: Cardiovascular diseases (CVDs) are leading cause of death and primary cause of morbidity and mortality in diabetic population. Epicardial adipose tissue (EAT) covers the heart's surface and is a source of biomolecules regulating heart and blood vessel physiology. The protective activation of the unfolded protein response (UPR) and autophagy allows the cardiomyocyte reticular network to restore energy and/or nutrient homeostasis and to avoid cell death. However, an excessive or prolonged UPR activation can trigger cell death. UPR activation is an early event of diabetic cardiomyopathies and deregulated autophagy is associated with CVDs. Results: An upregulation of UPR markers (glucose-regulated protein 78 KDa, glucose-regulated protein 94 KDa, inositol-requiring enzyme l alpha, protein kinase RNA-like ER kinase and CCAAT/enhancer-binding protein homologous protein (CHOP) gene) in EAT compared to subcutaneous adipose tissue (SAT), was observed as well as the UPR-related apoptosis marker caspase-4/procaspase-4 ratio but not in CHOP protein levels. Additionally, levels of ubiquitin and ubiquitinated proteins were decreased in EAT. Moreover, upregulation of autophagy markers (5' adenosine monophosphate-activated protein kinase, mechanistic target of rapamycin, Beclin 1, microtubule-associated protein light chain 3-II, lysosome-associated membrane protein 2, and PTEN-induced putative kinase 1) was observed, as well as an increase in the apoptotic Bim but not the ratio between Bim and the anti-apoptotic Bc1-2 in EAT. Diabetic patients show alterations in UPR activation markers but not in autophagy or apoptosis markers. Conclusion: UPR and autophagy are increased in EAT compared to SAT, opening doors to the identification of early biomarkers for cardiomyopathies and novel therapeutic targets.

【 授权许可】

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