Plasma based targeted metabolomic analysis reveals alterations of phosphatidylcholines and oxidative stress markers in guinea pig model of allergic asthma

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1866

Article

Kertys, Martin^1,2  Grendar, Marian²  Kosutova, Petra^2,3  Mokra, Daniela³  Mokry, Juraj¹ 

[1] Comenius Univ, Jessenius Fac Med Martin, Dept Pharmacol, Mala Hora 4C, Martin 03601, Slovakia

[2] Comenius Univ, Jessenius Fac Med Martin, Biomed Ctr Martin, Martin, Slovakia

[3] Comenius Univ, Jessenius Fac Med Martin, Dept Physiol, Martin, Slovakia

关键词: Allergic inflammation;    Animal model;    Asthma;    Guinea pigs;    Plasma metabolome;    Targeted metabolomics;   

DOI  :  10.1016/j.bbadis.2019.165572

来源: Elsevier

【 摘 要 】

Bronchial asthma is one of the most common, chronic respiratory diseases, characterized by reversible airway obstruction, eosinophil and Th2 infiltration, airway hyperresponsiveness and airway remodelling; with many cells and mediators involved. Metabolomics is a relatively new field in omics sciences enabling the identification of metabolome for better diagnostics and studying of diseases phenotype. The aim of this study was to investigate the role of targeted metabolomics study for better understanding of the bronchial asthma pathophysiology and finding potential biomarkers in experimental models of eosinophilic inflammation. Plasma level of 185 metabolites was measured with the AbsoluteIDQ (TM) p180 kit in guinea pigs with experimentally-induced allergic inflammation (n = 15) compared to naive non-sensitised and non-challenged controls (n = 18). Of the 185 metabolites identified in plasma, 22 were significantly different and changed in ovalbumin sensitised animals. Plasma level of 13 phosphatidylcholines with saturated and unsaturated long-chain fatty acids, total phosphatidylcholines count, carnitine, symmetric dimethylarginine and its ratio to total unmodified arginine, and kynurenine to tryptophan ratio were found to be decreased, while phospholipase A2 activity indicator, tryptophan, taurine and ratio of methionine sulfoxide to unmodified methionine were found to be increased in sensitised guinea pigs compared to naive controls. Targeted metabolomic analysis revealed significant differences in plasma metabolome of sensitised guinea pigs. Our observations point to the activation of inflammatory and immune pathways, as well as the involvement of oxidative stress.

【 授权许可】

Free